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tm7sf2 vector  (Addgene inc)


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    Addgene inc tm7sf2 vector
    A single amino acid mutation in TXNIP protein alters cholesterol pathway and insulin signaling genes in mouse livers. A , KEGG pathway analysis of WT, TXNIP C247S, and TXNIP KO groups’ liver gene expression fold-change comparisons (log2-fold expression) from bulk RNA-Seq experiments (n = 5, significance was determined as FDR ≤ 0.1). B , cholesterol biosynthesis pathway (upregulated genes in C247S mice livers are highlighted in yellow ), created with BioRender, ( C ) qPCR validation of upregulated cholesterol biosynthesis pathway genes in a different cohort of mouse livers (n = 6). Liver mRNA expressions of ( D ) <t>Tm7sf2</t> and ( E ) Irs2 in HFD-fed WT, TXNIP C247S, and TXNIP KO mice that are on HFD for 8 weeks (n = 10). F , liver mRNA expressions of Irs1 in HFD-fed WT, TXNIP C247S, and TXNIP KO mice that are on HFD for 8 weeks (n = 6). Liver mRNA expressions of ( G ) Tm7sf2 and ( H ) Irs2 in chow-fed WT, TXNIP C247S, and TXNIP KO mice (n = 6). I , liver mRNA expressions of Pck1 in HFD-fed WT and TXNIP C247S mice that are on HFD for 8 weeks (n = 6). Data represented as mean ± standard deviation, one-way ANOVA with Tukey post hoc tests were used, ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0005, and ∗∗∗∗ p < 0.00005. C247S, Cysteine 247 for Serine; FDR, false discovery rate; KEGG, Kyoto Encyclopedia of Genes and Genomes; qPCR, quantitative PCR; TXNIP, Thioredoxin-Interacting Protein.
    Tm7sf2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp-n2+vector/NET47+pEGFP-N2+(593)+(Plasmid+%2361986)/pmc12180993-233-6-17
    Average 93 stars, based on 1 article reviews
    tm7sf2 vector - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Covalent binding of thioredoxin to TXNIP is required for diet-induced insulin resistance in the liver"

    Article Title: Covalent binding of thioredoxin to TXNIP is required for diet-induced insulin resistance in the liver

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2025.110214

    A single amino acid mutation in TXNIP protein alters cholesterol pathway and insulin signaling genes in mouse livers. A , KEGG pathway analysis of WT, TXNIP C247S, and TXNIP KO groups’ liver gene expression fold-change comparisons (log2-fold expression) from bulk RNA-Seq experiments (n = 5, significance was determined as FDR ≤ 0.1). B , cholesterol biosynthesis pathway (upregulated genes in C247S mice livers are highlighted in yellow ), created with BioRender, ( C ) qPCR validation of upregulated cholesterol biosynthesis pathway genes in a different cohort of mouse livers (n = 6). Liver mRNA expressions of ( D ) Tm7sf2 and ( E ) Irs2 in HFD-fed WT, TXNIP C247S, and TXNIP KO mice that are on HFD for 8 weeks (n = 10). F , liver mRNA expressions of Irs1 in HFD-fed WT, TXNIP C247S, and TXNIP KO mice that are on HFD for 8 weeks (n = 6). Liver mRNA expressions of ( G ) Tm7sf2 and ( H ) Irs2 in chow-fed WT, TXNIP C247S, and TXNIP KO mice (n = 6). I , liver mRNA expressions of Pck1 in HFD-fed WT and TXNIP C247S mice that are on HFD for 8 weeks (n = 6). Data represented as mean ± standard deviation, one-way ANOVA with Tukey post hoc tests were used, ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0005, and ∗∗∗∗ p < 0.00005. C247S, Cysteine 247 for Serine; FDR, false discovery rate; KEGG, Kyoto Encyclopedia of Genes and Genomes; qPCR, quantitative PCR; TXNIP, Thioredoxin-Interacting Protein.
    Figure Legend Snippet: A single amino acid mutation in TXNIP protein alters cholesterol pathway and insulin signaling genes in mouse livers. A , KEGG pathway analysis of WT, TXNIP C247S, and TXNIP KO groups’ liver gene expression fold-change comparisons (log2-fold expression) from bulk RNA-Seq experiments (n = 5, significance was determined as FDR ≤ 0.1). B , cholesterol biosynthesis pathway (upregulated genes in C247S mice livers are highlighted in yellow ), created with BioRender, ( C ) qPCR validation of upregulated cholesterol biosynthesis pathway genes in a different cohort of mouse livers (n = 6). Liver mRNA expressions of ( D ) Tm7sf2 and ( E ) Irs2 in HFD-fed WT, TXNIP C247S, and TXNIP KO mice that are on HFD for 8 weeks (n = 10). F , liver mRNA expressions of Irs1 in HFD-fed WT, TXNIP C247S, and TXNIP KO mice that are on HFD for 8 weeks (n = 6). Liver mRNA expressions of ( G ) Tm7sf2 and ( H ) Irs2 in chow-fed WT, TXNIP C247S, and TXNIP KO mice (n = 6). I , liver mRNA expressions of Pck1 in HFD-fed WT and TXNIP C247S mice that are on HFD for 8 weeks (n = 6). Data represented as mean ± standard deviation, one-way ANOVA with Tukey post hoc tests were used, ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0005, and ∗∗∗∗ p < 0.00005. C247S, Cysteine 247 for Serine; FDR, false discovery rate; KEGG, Kyoto Encyclopedia of Genes and Genomes; qPCR, quantitative PCR; TXNIP, Thioredoxin-Interacting Protein.

    Techniques Used: Mutagenesis, Gene Expression, Expressing, RNA Sequencing, Biomarker Discovery, Standard Deviation, Real-time Polymerase Chain Reaction

    Elevated Tm7sf2 gene expression can improve insulin signaling in human HEPG2 cells under redox stress. A and B , Akt Ser473 phosphorylation and total Akt levels of empty vector (EV) or TM7SF2-transfected, insulin-stimulated HEPG2 cells with or without H 2 O 2 stimulation (n = 4). C – F , mRNA expressions of IRS2, IRS1, G6PC, and PCK1 genes in EV or TM7SF2-transfected, insulin-stimulated HEPG2 cells with or without H 2 O 2 stimulation (n = 6). G and H , PEPCK/PCK1 protein levels of EV or TM7SF2-transfected, insulin-stimulated HEPG2 cells with or without H 2 O 2 stimulation (n = 4). Data represented as mean ± standard deviation, one-way ANOVA with Tukey or Bonferroni post hoc tests, Kruskal–Wallis, and Student t tests were used, ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0005, and ∗∗∗∗ p < 0.00005.
    Figure Legend Snippet: Elevated Tm7sf2 gene expression can improve insulin signaling in human HEPG2 cells under redox stress. A and B , Akt Ser473 phosphorylation and total Akt levels of empty vector (EV) or TM7SF2-transfected, insulin-stimulated HEPG2 cells with or without H 2 O 2 stimulation (n = 4). C – F , mRNA expressions of IRS2, IRS1, G6PC, and PCK1 genes in EV or TM7SF2-transfected, insulin-stimulated HEPG2 cells with or without H 2 O 2 stimulation (n = 6). G and H , PEPCK/PCK1 protein levels of EV or TM7SF2-transfected, insulin-stimulated HEPG2 cells with or without H 2 O 2 stimulation (n = 4). Data represented as mean ± standard deviation, one-way ANOVA with Tukey or Bonferroni post hoc tests, Kruskal–Wallis, and Student t tests were used, ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0005, and ∗∗∗∗ p < 0.00005.

    Techniques Used: Gene Expression, Phospho-proteomics, Plasmid Preparation, Transfection, Standard Deviation

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Characterization of myoinhibitory peptide signaling system and its implication in larval metamorphosis and spawning behavior in Pacific abalone.
    Article Snippet: Myoinhibitory peptides (MIPs) affect various physiological functions, including juvenile hormone signaling, muscle contraction, larval development, and reproduction in invertebrates.. Although MIPs are ligands for MIP and/or sex peptide receptors (MIP/SPRs) in diverse arthropods and model organisms belonging to Lophotrochozoa, the MIP signaling system has not yet been fully investigated in mollusks.. In this study, we identified the MIP signaling system in the Pacific abalone Haliotis discus hannai (Hdh).

    Clone Assay:

    Article Title: Characterization of myoinhibitory peptide signaling system and its implication in larval metamorphosis and spawning behavior in Pacific abalone.
    Article Snippet: Myoinhibitory peptides (MIPs) affect various physiological functions, including juvenile hormone signaling, muscle contraction, larval development, and reproduction in invertebrates.. Although MIPs are ligands for MIP and/or sex peptide receptors (MIP/SPRs) in diverse arthropods and model organisms belonging to Lophotrochozoa, the MIP signaling system has not yet been fully investigated in mollusks.. In this study, we identified the MIP signaling system in the Pacific abalone Haliotis discus hannai (Hdh).

    Plasmid Preparation:

    Article Title: Characterization of myoinhibitory peptide signaling system and its implication in larval metamorphosis and spawning behavior in Pacific abalone.
    Article Snippet: Myoinhibitory peptides (MIPs) affect various physiological functions, including juvenile hormone signaling, muscle contraction, larval development, and reproduction in invertebrates.. Although MIPs are ligands for MIP and/or sex peptide receptors (MIP/SPRs) in diverse arthropods and model organisms belonging to Lophotrochozoa, the MIP signaling system has not yet been fully investigated in mollusks.. In this study, we identified the MIP signaling system in the Pacific abalone Haliotis discus hannai (Hdh).



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    Image Search Results


    A single amino acid mutation in TXNIP protein alters cholesterol pathway and insulin signaling genes in mouse livers. A , KEGG pathway analysis of WT, TXNIP C247S, and TXNIP KO groups’ liver gene expression fold-change comparisons (log2-fold expression) from bulk RNA-Seq experiments (n = 5, significance was determined as FDR ≤ 0.1). B , cholesterol biosynthesis pathway (upregulated genes in C247S mice livers are highlighted in yellow ), created with BioRender, ( C ) qPCR validation of upregulated cholesterol biosynthesis pathway genes in a different cohort of mouse livers (n = 6). Liver mRNA expressions of ( D ) Tm7sf2 and ( E ) Irs2 in HFD-fed WT, TXNIP C247S, and TXNIP KO mice that are on HFD for 8 weeks (n = 10). F , liver mRNA expressions of Irs1 in HFD-fed WT, TXNIP C247S, and TXNIP KO mice that are on HFD for 8 weeks (n = 6). Liver mRNA expressions of ( G ) Tm7sf2 and ( H ) Irs2 in chow-fed WT, TXNIP C247S, and TXNIP KO mice (n = 6). I , liver mRNA expressions of Pck1 in HFD-fed WT and TXNIP C247S mice that are on HFD for 8 weeks (n = 6). Data represented as mean ± standard deviation, one-way ANOVA with Tukey post hoc tests were used, ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0005, and ∗∗∗∗ p < 0.00005. C247S, Cysteine 247 for Serine; FDR, false discovery rate; KEGG, Kyoto Encyclopedia of Genes and Genomes; qPCR, quantitative PCR; TXNIP, Thioredoxin-Interacting Protein.

    Journal: The Journal of Biological Chemistry

    Article Title: Covalent binding of thioredoxin to TXNIP is required for diet-induced insulin resistance in the liver

    doi: 10.1016/j.jbc.2025.110214

    Figure Lengend Snippet: A single amino acid mutation in TXNIP protein alters cholesterol pathway and insulin signaling genes in mouse livers. A , KEGG pathway analysis of WT, TXNIP C247S, and TXNIP KO groups’ liver gene expression fold-change comparisons (log2-fold expression) from bulk RNA-Seq experiments (n = 5, significance was determined as FDR ≤ 0.1). B , cholesterol biosynthesis pathway (upregulated genes in C247S mice livers are highlighted in yellow ), created with BioRender, ( C ) qPCR validation of upregulated cholesterol biosynthesis pathway genes in a different cohort of mouse livers (n = 6). Liver mRNA expressions of ( D ) Tm7sf2 and ( E ) Irs2 in HFD-fed WT, TXNIP C247S, and TXNIP KO mice that are on HFD for 8 weeks (n = 10). F , liver mRNA expressions of Irs1 in HFD-fed WT, TXNIP C247S, and TXNIP KO mice that are on HFD for 8 weeks (n = 6). Liver mRNA expressions of ( G ) Tm7sf2 and ( H ) Irs2 in chow-fed WT, TXNIP C247S, and TXNIP KO mice (n = 6). I , liver mRNA expressions of Pck1 in HFD-fed WT and TXNIP C247S mice that are on HFD for 8 weeks (n = 6). Data represented as mean ± standard deviation, one-way ANOVA with Tukey post hoc tests were used, ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0005, and ∗∗∗∗ p < 0.00005. C247S, Cysteine 247 for Serine; FDR, false discovery rate; KEGG, Kyoto Encyclopedia of Genes and Genomes; qPCR, quantitative PCR; TXNIP, Thioredoxin-Interacting Protein.

    Article Snippet: Cells were transfected with EV or Tm7sf2 vector (NET47 pEGFP-N2 [593] was a gift from Eric Schirmer, Addgene plasmid #61986) using Lipofectamine 3000 reagent (Thermo Fisher).

    Techniques: Mutagenesis, Gene Expression, Expressing, RNA Sequencing, Biomarker Discovery, Standard Deviation, Real-time Polymerase Chain Reaction

    Elevated Tm7sf2 gene expression can improve insulin signaling in human HEPG2 cells under redox stress. A and B , Akt Ser473 phosphorylation and total Akt levels of empty vector (EV) or TM7SF2-transfected, insulin-stimulated HEPG2 cells with or without H 2 O 2 stimulation (n = 4). C – F , mRNA expressions of IRS2, IRS1, G6PC, and PCK1 genes in EV or TM7SF2-transfected, insulin-stimulated HEPG2 cells with or without H 2 O 2 stimulation (n = 6). G and H , PEPCK/PCK1 protein levels of EV or TM7SF2-transfected, insulin-stimulated HEPG2 cells with or without H 2 O 2 stimulation (n = 4). Data represented as mean ± standard deviation, one-way ANOVA with Tukey or Bonferroni post hoc tests, Kruskal–Wallis, and Student t tests were used, ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0005, and ∗∗∗∗ p < 0.00005.

    Journal: The Journal of Biological Chemistry

    Article Title: Covalent binding of thioredoxin to TXNIP is required for diet-induced insulin resistance in the liver

    doi: 10.1016/j.jbc.2025.110214

    Figure Lengend Snippet: Elevated Tm7sf2 gene expression can improve insulin signaling in human HEPG2 cells under redox stress. A and B , Akt Ser473 phosphorylation and total Akt levels of empty vector (EV) or TM7SF2-transfected, insulin-stimulated HEPG2 cells with or without H 2 O 2 stimulation (n = 4). C – F , mRNA expressions of IRS2, IRS1, G6PC, and PCK1 genes in EV or TM7SF2-transfected, insulin-stimulated HEPG2 cells with or without H 2 O 2 stimulation (n = 6). G and H , PEPCK/PCK1 protein levels of EV or TM7SF2-transfected, insulin-stimulated HEPG2 cells with or without H 2 O 2 stimulation (n = 4). Data represented as mean ± standard deviation, one-way ANOVA with Tukey or Bonferroni post hoc tests, Kruskal–Wallis, and Student t tests were used, ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0005, and ∗∗∗∗ p < 0.00005.

    Article Snippet: Cells were transfected with EV or Tm7sf2 vector (NET47 pEGFP-N2 [593] was a gift from Eric Schirmer, Addgene plasmid #61986) using Lipofectamine 3000 reagent (Thermo Fisher).

    Techniques: Gene Expression, Phospho-proteomics, Plasmid Preparation, Transfection, Standard Deviation